纳洛酮对酒精性脂肪肝大鼠作用探讨

To Explore the Effect of Naloxone on Alcoholic Fatty Liver in Rats

目的 探讨纳洛酮对酒精性脂肪肝的作用及其可能机制。方法 将 48只Wistar雄性大鼠,随机分为 4组:酒精性脂肪肝模型组、纳洛酮低剂量处理组、纳洛酮高剂量处理组,正常对照组。4周末,处死所有大鼠,检测血液中天门冬氨酸氨基转移酶 (AST)、丙氨酸氨基转移酶(ALT)、谷光甘肽-S转移酶(GST)和β 内啡肽(β EP)含量。同时行肝脏病理组织学检查。结果 纳洛酮处理组血浆AST、ALT、GST和β EP含量明显低于模型组(P<0 05 ),但又高于正常对照组 (P<0 05 ),且处理组内部相比,差异也有显著性 (P<0 05)。病理检查发现处理组肝脏脂肪变程度明显轻于模型组。结论 纳洛酮可以通过降低血浆中β EP水平而具有防治酒精性脂肪肝的作用。

Objective To explore the effect of naloxone on alcoholic fatty liver (AFL) in rats and its possible mechanism.Methods 48 Wistar male rats were divided randomly into four groups:Model group,naloxone low dose group,naloxone high dose group and normal control group.By the end of the fourth week,all rats were weighted,narcosised,sacrificed,and got the whole liver.Level of β-endorphin (β-EP),aspartate aminotransferase (AST),alanine aminotransferase (ALT) and glutathione s-transferasc (GST) in plasma were measured.Liver sample were analyzed for histopathology with microscope.Results Rat models of AFL were established successfully.Contrast with normal control group,the level of β-EP,AST,ALT,GST in plasma increased in model group and decreased in treatment group markedly (P<0.01).Classic hepatocyte fatty degeneration were observed in model group.Conclusion Long-term alcohol and excessive drinking can increase the level of β-endorphin (β-EP) in plasma; naloxone can decrease β-EP level in plasma and prevent the formation of AFL.