三角帆蚌十六种同工酶系统的表型及其在瘟病病蚌中的病理变化

SIXTEEN ISOZYMIC PHENOTYPES OF HYRIOPSIS CUMINGII LEA AND THEIR PATHOLOGICAL CHANGES IN PLAGUE DISEASED MUSSELS

采用聚丙烯酰胺凝胶电泳技术,对我国三角帆蚌(Hyriopsis cumingii Lea)11种组织中16种同工酶系统的酶谱表型、组织分布、活性含量和迁移特征进行了分析,并就不同酶谱特征与基因表达状况作了讨论。证实酯酶和α-磷酸甘油脱氢酶的表型和活性含量在病蚌体内有明显的紊乱现象,表明三角帆蚌瘟病的病理机制与消化系统脂类代谢异常有密切关系。消化系统两种酶谱的特异性变化,可作为瘟病早期诊断的生化辅助指标。

Sixteen isozymes, extracted from eleven tissues of healihy Hyriopsis cumingii Lea mussels and plague-diseased mussels respectively,were analysed with vertical slab polyacrylamide gel electrophoresis. The isozymes referred above were alcohol dehydrogenase (ADH), α-phosphoglycerol dehydrogenase (α-GPDH), lactate dehydrogenase(LDH). malate dehydrogenase (MDH). malic enzyme(ME), isocitrate dehydrogenase (IDH),6-phosphogluconate dehydrogenase(6PGD), glucose-6-phosphate dehydrogenase (G6PD), catalase, peroxidase (POD), cytochrome oxidase, glutamatepyruvate transaminase (GPT), alkaline phosphatase (AKP), esterase (EST), amylase and adenosine triphosphatease (ATPase). The isozymic phenotypes, tissue distributions, relative activities and mobile characteristics of the sixteen isozymes were determined, and the possible relationships between the isozyme pattern and gene expression were discussed. In comparison with the healthy mussels, the zymograms and activities of EST and α-GPDH in diseased mussels were evidently disordered. It provided the biochemical evidence that the mechanism of plague pathogenesis was closely related to the lipid metabolic block in digestive system of the diseased mussels. The pathological changes of the two isozymes in digestive system were considered to be an auxiliary biochemical diagnosis marker in the early stage of Hyriopsis cumingii Plague.