大阪鲫卵黄蛋白原和钙离子关系的初步研究

THE INITIAL STUDY ON THE RELATIONSHIP BETWEEN VITELLOGENIN AND CALCIUM ION IN CARASSIUS AURATUS CUVIERI

注射苯甲酸雌二醇诱导雄性大阪鲫产生雌性特异血清蛋白(卵黄蛋白原),随着诱导天数的增加,血清中的钙离子浓度升高,同时血清中的卵黄蛋白原的含量也相应增加。在此过程中肝细胞的细胞质中存在大量的钙离子,并进行着旺盛的蛋白合成。雌鱼和被诱导的雄鱼一样在肝细胞内也存在大量的钙离子。正常雄鱼的肝细胞不合成卵黄蛋白原,只有很少的钙离子存在。腹腔注射氯化钙使卵母细胞的卵径增大(实验组为 1.08±0.14mm;对照组为 1.04±0.14mm),并使卵母细胞的蛋白含量升高,同时卵泡膜上毛细血管的分布更为稠密,这表明钙离子可促进卵母细胞的发育和蛋白的吸收。

Adminstration of massion doses of estradiol benzoate into malefish caused an increase of vitellogenin and caleium levels in serum and prolifica-tion of rough endoplasmic reticulum and mitochondria in liver cells. Meanwhile,a lot of calcium presented in liver cell's cytoplasm. There was also calcium inthe liver cell of naturally matural female fish. But the liver cells of the male fishwithout injection of estrodiol benzoate had few calcium. This suggested that cal-cium had some effects on the synthesis of vitellogenin, The injection intraperi-toneally with calcium chloride caused the expansion of egg's diamater and increa- se of protein content in oocytes. When the female fish was injected with calciumchloride,the diameter of oocytes was 1.08±0.14mm(n=60)and the protein content was29.14%. As to the control,thes relevant data were 1.04±0.14mm (n=60)and 14.60%.The capillary onfollicular membrance with calcium chloride was dencer than con-trol. This indicated that calcium could enhance the growth of oocytes and theabsorption of protein, especially vitellogenin.