摘要
建立了HPLC法测定野菊花中蒙花苷含量的方法。采用AICHROMC18(2 5 0mm× 4 6mm ,5 μm)色谱柱 ,以甲醇— 0 8%磷酸 (9∶7)为流动相 ,流速为 1 2ml·min- 1,检测波长为 32 6nm。蒙花苷在 0 0 4 3～ 2 16 μg的范围内具有良好的线性关系 ,相关系数为 0 9999,平均回收率为 98 9% (RSD为1 8% )。本方法操作简便 ,灵敏度高 ,结果准确可靠。
A HPLC method for the determination of buddleoside in Ch rysanthemum Indicum L. in was established. AICHROM C 18column (250mm×4.6 mm,5μm) was used with methanol-0.8% phosphoric acid (9∶7) as mobile phase. Th e flow rate was 1.2ml·min -1and detecting wavelength 326nm. The linear r ange was 0.043～2.16μg, γ=0.9999. The average recovery rate was 98.9% ( RSD=1.8%). The method was simple, sensitive and accurate.
出处
《中国药事》
CAS
2005年第2期97-98,共2页
Chinese Pharmaceutical Affairs
关键词
HPLC
野菊花
蒙花苷
HPLC
Buddleoside
Chrysanthemum indicum L.
