重症肌无力患者外周血单个核细胞Fas及血清sFas、TNFα检测

Detection of Fas on peripheral blood mononulear cell membrane and the serum soluble Fas and TNFα of the patients with myasthenia gravis

目的:探讨外周血淋巴细胞凋亡与重症肌无力(MG)发生的关系。方法: 收集 20例MG患者及 20例健康献血者的外周血,分离单个核细胞和血清,用流式细胞仪测定单个核细胞膜Fas,用双抗体夹心ELISA法测定血清中sFas、TNFα水平。结果:MG患者血清sFas、TNFα水平较对照组高(P<0. 01);单个核细胞表面Fas分子的表达率低于对照组(P<0. 05)。结论:MG患者单个核细胞膜Fas分子和血清sFas、TNFα水平异常,可能与疾病的发生有关。

Aim: To investigate the relationship of lymphocyte apoptosis and myasthenia gravis(MG).Methods: The serum and peripheral blood mononuclear cells(PBMC) were isolated from 20 patients with MG and 20 normal people, respectively. Fas molecule on PBMC surface was analyzed by flow cytometry. The serum soluble Fas(sFas) and TNFα were detected by ELISA.Results: Compared with the normal control, the level of serum sFas and TNFα of MG patients were significantly higher (P<0.01),and Fas expression on PBMC surface was lower (P<0.05). Conclusion: The abnormal changes of Fas molecule, serum sFas, and TNFα may be associated with the genesis of MG.