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大肠腺癌组织中凝血酶敏感蛋白-1和微血管密度检测

Detection of thrombospondin-1 and microvessel density in colorectal adenocarcinoma tissue
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摘要 　　　目的:探讨大肠腺癌组织中凝血酶敏感蛋白 1 (thrombospondin 1, TSP- 1)的表达及其临床意义。方法:利用免疫组织化学SP法检测 45例大肠腺癌组织中TSP- 1的表达及微血管密度 (microvesseldensity, MVD),并以15例正常大肠黏膜组织为对照。结果:正常大肠黏膜组织中未见TSP- 1阳性表达;大肠腺癌组织中TSP- 1的阳性率为 42. 2% (19 /45)。TSP- 1阳性组MVD为(14. 37±4. 86 ),低于TSP- 1阴性组的 ( 29. 80±6. 35 ) (t=2. 735,P<0. 01);TSP- 1的阳性表达率随肿瘤Dukes分期的增加而降低(χ2 =4. 62,P<0. 05),与MVD呈负相关 (r=-0. 783,P<0. 01)。结论:TSP 1的阳性表达抑制了大肠癌新生血管的形成,从而阻止其发展及转移。 Aim: To investigate the expression of thrombospondin-1 (TSP-1) in colorectal carcinoma and its clinical significance. Methods: Using immunohistochemical technique, MVD and TSP-1 expression in 45 cases of colorectal adenocarcinoma and 15 cases of normal colorectal mucosa were detected. Results:There was no expression of TSP-1 in normal colorectal tissue.The positive rate of TSP-1 in colorectal adenocarcinoma tissue was 42.2%(19/45),which decreased with Dukes stage (χ2=4.62,P<0.05).MVD in TSP-1 positive group(14.37±4.86) was significantly lower than that in the negative group (29.80±6.35)(t=2.735,P<0.01), and the expression of TSP-1 had a negative correlation with MVD (r=-0.783, P<0.01). Conclusion: TSP-1 may inhibit the angiogenesis,and prevent from the vessels development and metastasis of colorectal carcinoma.
作者 李向阳 刘晓辉 李恩君 刘争 僧国珍
机构地区 郑州大学第一附属医院普外科 南阳市中医院医教科
出处 《郑州大学学报（医学版）》 CAS 北大核心 2005年第1期119-121,共3页 Journal of Zhengzhou University(Medical Sciences)
关键词 凝血酶敏感蛋白-1 大肠肿瘤 微血管密度 thrombospondin-1 colorectal neoplasm microvessel density
分类号 R735.3 [医药卫生—肿瘤]
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参考文献9

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郑州大学学报（医学版）
2005年 第1期

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