子宫与卵巢异位内膜细胞凋亡和增殖特性的研究

Research on the apoptosis and proliferation features in ectopic endometrial cells of uterus and ovary

目的 探讨子宫腺肌病与卵巢异位囊肿异位内膜的细胞凋亡和增殖特性及发病机制。方法 2 0 0 2年 6月至 2 0 0 3年 6月南方医科大学珠江医院采用免疫组化S P法 ,检测子宫腺肌病 (46例 )和卵巢异位囊肿 (6 0例 )的在位及异位子宫内膜中凋亡调控基因蛋白bcl 2、bax及细胞增殖标记物Ki 6 7蛋白的表达。结果 bcl 2蛋白、bax蛋白及Ki 6 7蛋白在两症的在位内膜以及子宫腺肌病异位内膜中均呈现周期性改变 ,而bcl 2蛋白及Ki 6 7蛋白在卵巢异位囊肿异位内膜中呈持续性增强 ,较在位内膜差异有显著性意义 (P <0 0 5 )。结论 子宫腺肌病异位内膜的细胞凋亡和增殖受卵巢性激素周期性的调节 ,卵巢异位囊肿异位内膜则相反 ,两者细胞凋亡和增殖特性有着明显的不同 ,是两种不同的疾病。

Objective To investigate the property of apoptosis and proliferation of the ectopic endometrial cells in adenomyosis and ovarian ectopic cyst as well as their possible pathogenesis. Method To detect the expression of the apoptotic protein bcl-2?bax and the cell proliferating labeling antigen ki-67 in eutopic and ectopic endometrial cells of adenomyosis and ovarian ectopic cyst with the immunohistochemical S-P method. Result Protein bcl-2?bax and Ki-67 cyclically changed both in the eutopic and the ectopic endometrial cells of adenomyosis and ovarian cyst;while protein bcl-2 and Ki-67 of ovarian ectopic cyst were continuously enhanced,which was significantly different from that of eutopic endometrium (P<0.05). Conclusion The apoptosis and proliferation of the ectopic endometrial cells in adenomyosis are cyclically adjusted by ovary, while those in ovarian ectopic cyst are contrary to them, either of which has its own origin.