大豆体细胞组织培养再生植株的研究——Ⅰ 培养基、基因型、植物激素对诱导大豆再生植株的影响

A STUDY OF SOYBEAN PLANT REGENERATION VIA SOMATIC TISSURE CULTURES

本研究以野生大豆(Glycine soja Sieb et Zucc.)10品系、半野生大豆(Glycine gracilis Skv.)11品系、栽培大豆(Glycine max (L.)Merril)46品种(系)和5个杂种后代为材料,通过组织培养方法,研究了大豆体细胞组织再生植株的主要影响因素。并以器官分化和体细胞胚两种不同方式再生完整大豆植株。

The studies of soybean regeneration plant via callus cultures from a wild variety (Glycine gracilis Skv.) is reported here. The main factors which influence organogenesis are also discussed in this paper. They are the basal media, genotypes and phytohormone. The regeneration plants could be produced from this wild sub-variety through organogenesis by culturing on a series of appropriate media. MS + 2,4-D 5.0 ppm+kinetin 1.0 ppm was an effective medium in inducing qualified calluses.A series of appropriate media in plant regeneration were founded and the regeneration plants were obtained. According to the information available now, the study of soybean regeneration plants from G. gracilis Skv. has not yet been reported before this.