摘要
建立了以茜素红(AR)为电活性探针方波伏安法间接测定无电活性的壳聚糖(CS)的新方法。在0 1mol/LHAc＿NaAc(pH4 5)缓冲溶液中,AR与CS＿AR在GCE上均能形成一准可逆的吸附伏安峰(Vpc=-0 46V,Vpa=-0 41V)。实验表明,CS在低浓度(1 25×10-8～2 5×10-7mol/L)和高浓度(2 5×10-7～1 25×10-5mol/L)的条件下对体系的峰电流分别具有线性增敏和减敏效应。采用标准加入法测定了不同样品中壳聚糖的含量,其回收率在97 33%～102 00%之间,结果满意。
A new method to indirectly determine chitosan(CS) was established by using alizarin red(AR) as an electrochemical activity probe and square wave voltammetry. In 0.1 mol/L HAc_NaAc buffer solution(pH 4.5), AR and AR_CS complexis can be easily reduced or oxided on the glass carbon electrode(GCE) and they have a well_defined quasi_revesible wave(V_(pc)=-0.45 V,V_(pa)=-0.41 V) with adsorption characteristics. The increase and decrease of reductive peak current of AR are proportional to CS concentration in the range of 1.25×10^(-8)～2.5×10^(-7) mol/L and 2.5×10^(-7)～1.25×10^(-5) mol/L, respectively. The method has been successfully used for the determination of CS in different samples by standard addition. The recovery was measured to be 97.33%～102.00%.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第1期129-131,共3页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
广西壮族自治区自然科学基金资助项目(0141037
0342018)
关键词
壳聚糖
茜素红
方波伏安法
测定
chitosan
alizarin red
square wave voltammetry
determination
