光动力学疗法诱导人膀胱癌细胞凋亡的实验研究

Inducing Apoptosis of Human Bladder Cancer Cell In Vitro by Chlorophyll Derivative-Photodynamic Therapy

目的 :联合应用流式细胞仪和激光共聚焦显微镜研究叶绿素衍生物光动力学疗法诱导人膀胱癌细胞 (T - 2 .4andSCaBER)凋亡的现象。 方法 :光动力疗法体外作用于人膀胱癌T - 2 4andSCaBER细胞 ,细胞凋亡指数采用双免疫荧光染色(AnnexinV/PI)和流式细胞仪技术测定 ,凋亡细胞的形态学研究采用AO/EB双染色和激光共聚焦显微镜技术。 结果 :经光动力学作用后的膀胱癌细胞通过诱导凋亡而被抑制了细胞的活性。细胞被阻滞于G0～G1期。 结论 :叶绿素衍生物光动力学疗法通过诱导凋亡而抑制膀胱肿瘤的生长 ,激光共聚焦显微镜能提供更可靠的细胞早期凋亡的影像学资料。

Objective To investigate the effect of Chlorophyll derivative-photodynamic therapy(CPD4-PDP)on human bladder cancer cell(T-24 and SCaBER cell) Methods Human bladder cancer cells were used as an in vitro model.Cells were treated with PDP.The apoptotic index of terazosin on PC-3 cells were assessed by two-color immunofluorescent staining(Annexin V/PI)and FCM.Apoptotic cell morphology was assessed by CLSM after AO/EB staining with combined use of flow cytometry(FCM) and confocal lasser scanning microscopy(CLSM).Results The treatment of bladder cancer cells with PDP resulted in a significant increase of cell apoptosis.CPD4-PDT effectively blocked tumor cell growth and arrested cells in the Go-G1 phase of the cell cycle. Conclusion These findings demonstrate the role of PDT to suppress bladder cell growth in vitro by inducing apoptosis.The CLSM can clearly demonstrate the occurrence of apoptosis,and provide much more reliable information for the early apoptosis.