缺血-再灌注损伤对视网膜明胶酶活性的影响

Expression of Gelatinase in Retinal Ischemia-reperfusion Injury

目的 :探讨大鼠视网膜缺血 再灌注 (RIR)损伤时明胶酶A(MMP 2 )和明胶酶B(MMP 9)蛋白的动态变化及意义。方法 :采用前房高压灌注法造成大鼠视网膜缺血 1h ,RIR 0 ,3,2 4 ,4 8和 72h后取材 ,用免疫组化SP法和计算机图像分析系统检测MMP 2 ,9的表达 ,以视网膜电图 (ERG)评估视网膜损伤情况。结果 :对照组和RIR0h视网膜MMP 2 ,9表达极弱 ,阳性染色于RIR 3h增加 ,2 4h达高峰 ,以后逐渐下降。ERGb波波幅于RIR 2 4h达最低 ,之后逐渐恢复。结论 :明胶酶是参与RIR损伤的重要因素。

Objective: To investigate the dynamic changes and significance of matrix metalloproteinases (MMPs), especially gelatinases (MMP-2 and -9) in an experimental retinal ischemia-reperfusion(RIR) injury. Methods: Ischemia injury was induced in adult rats by increasing the pressure of the anterior chamber with perfusion of normal saline. After one hour of ischemia and a varied reperfusion time (0, 3, 24, 48 or 72 h), the rats eyes were enucleated. The levels of MMP-2 and -9 were confirmed by immunohistochemical methods and analyzed by computer-picture analytic system. The retinal damage was assessed by Electroretinogram (ERG) recordings. Results: MMP-2 and -9 positive expression were rarely detected in the control or RIR 0 h specimens, while they increased at RIR 3 h, reached maximal levels at 24 h and dropped gradually afterwards. The b-wave amplitude reduced to the minimum at 24 h and then resumed. Conclusion: Gelatinase may play an important role in the pathomechamism of RIR injury.