摘要
采用 DNA 杂交的方法,对异育银鲫及其人工杂合种进行了外源 DNA 的检测分析,发现两个雌核发育系的异育银鲫及其人工杂合种的 DNA 与红鲤 DNA 片段间的分子杂交均有阳性斑点出现,这表明,在异育银鲫及其人工杂合种产生过程中,父本(红鲤)的 DNA 片段的确可以随机地掺入到母本细胞的 DNA 中,从而产生了异精效应,使其子代生长加快,并出现父本性状。
In this paper,the heterologous genetic materials in allogynogenetic crucian carp and its artificial hybrids were analysed by using the method of dot hybridization of DNA.There were some positive dots when the DNA in two clones of allogynogenetic crucian carp and their ar- tificial hybrids were crossed with the pieces of DNA in red carp.Based on this result,it was suggested that,in the breeding of the allogynogenetic crucian carp and its artificial hybrids, some pieces of paternal DNA can integrate to maternal DNA at random,resulting in the effect of allogynogenesis',i.e.the allogynogenetic offspring have higher growth rate and some paternal characteristics.
出处
《水生生物学报》
CAS
CSCD
北大核心
1993年第1期22-26,共5页
Acta Hydrobiologica Sinica
基金
国家自然科学基金(39170602)
湖北省自然科学基金(89J17)
国家淡水生态及生物技术开放实验室(FEBL)资助
关键词
人工杂合种
异育银鲫
遗传物质
Allogynogenetic crucian carp
Artificial hybrids of allogynogenetic crucian carp
DNA hybridization
Detection of paternal(red carp)DNA fragments
