野生纤毛虫同工酶微量等电聚焦分析探索

STUDY OF ESTERASE ISOZYMES IN WILD CARCHESIUM POLYPINUM BY MICRO-ISOELECTRIC FOCUSSING

微量电泳是用来分离和表征提取于少量生物材料中的核酸和蛋白质的分析技术。作者以缘毛目螅状独缩虫(Carchesium polypinum Linne,1758)为材料,用微量等电聚焦(Microisoelectric focussing)探索了分析野生纤毛虫同工酶的可行性。实验结果表明:(1) 样品量小至两个群体螅状独缩虫(约200个个体)即可进行酯酶同工酶微量等电聚焦分析;(2) 自野外采集材料中立即分离制备的螅状独缩虫匀浆上清液的酯酶同工酶酶谱与实验室内放置10h后分离制备的酶谱几乎一致。因此,野生纤毛虫同工酶可用微量等电聚焦进行分析。

Micro-electrophoreses are analytical techniques available for separating and characterizing RNAs, DNAs and proteins extracted from small amounts of biological materials. Microisoelectric focussing, one kind of micro-electrophoreses, is used for the first time to analyse esterase isozyme of the wild ciliate Carchesiurn polypinum(Linne, 1758), the isozymes of which have not been investigated before. The results of the experiments demonstrate: 1, samples of two colonies of Carchesium polypinum(about 200 zooids) are sufficient for the microfocussing analysis of esterase isozyme; 2, Carchesiurn polypinum separated immediately from the sample collected from Lake Donghu has almost the same pattern of esterase isozyme as the sample which has been kept in the laboratory for 10 hrs at 30℃. Therefore, isozymes of wild ciliates can be analysed by microfocussing.