长期不进展者与艾滋病患者HIV-1 nef特异性CD8^+ T细胞应答的初步研究

HIV-1 nef specific cytotoxic T lymphocyte responses in long-term nonprogressors and AIDS patients

目的 探讨我国HIV 1nef特异性CD8+T细胞应答的特点及其免疫保护作用。方法以长期不进展组 (LTNP) 7例和艾滋病组 9例为研究对象 ,以覆盖HIV 1nef全长的 2 6个重叠肽段组成的 3个肽段库作为刺激原 ,用IFN ELISPOT方法检测LTNP组与艾滋病组患者HIV 1nef特异性CD8+T细胞应答 ,并测定其CD4 +T细胞、CD8+T细胞和病毒载量 ,观察两组间HIV 1nef特异性CD8+T细胞应答的差异及其与CD4 +T细胞和病毒载量的相关性。结果 LTNP组和艾滋病组HIV 1nef特异性CD8+T细胞应答的强度分别为 4 0 4± 334和 5 9± 12 1SFC/ 10 6外周血单个核细胞 (PBMC) ,LTNP组显著高于艾滋病组 ;HIV 1nef特异性CD8+T细胞应答的强度与CD4 +T细胞计数呈正相关 ,但与病毒载量没有显著的相关性。结论 HIV 1nef特异性CD8+T细胞应答在艾滋病发病中具有一定的保护作用 。

Objective To investigate the HIV-1 nef specific CD8 + T cell responses in long-term nonprogressor (LTNP) and AIDS patient in China.Methods Peripheral blood mononuclear cells were collected from 7 LTNPs and 9 AIDS patients, 12 males and 4 females, aged 37 (27～55). A panel of 26 overlapping peptides spanning HIV-1 nef constituting 3 peptide pools was used as antigens. The HIV-1 nef specific CD8 + T cell responses of the LTNPs and AIDS patients were examined by IFN- ELISPOT assay. The amount of CD4 + T cell and viral load were detected. The relationships between HIV-1 nef specific CD8 + T cell responses and CD4 + T cell counts and viral loads were studied.Results The strength of HIV-1 nef specific CD8 + T cell responses of LTNP group was 404±33 SFC/10 6 PBMCs, significantly higher than that of the AIDS group (59±121 SFC/10 6 PBMCs ). The strength of HIV-1 nef specific CD8 + T cell responses was positively correlated with the CD4 + T cell count. No relationship between the strength of CD8 + T cell responses and viral load was found.Conclusion HIV-1 nef specific CD8 + T cell responses may play an important role in preventing disease progression of HIV infection. There is a cross immune response between the major HIV-1 strains in Europe and those in China .