黄连专属性对照物质及其指纹图谱的创建

Establishment of the Control Substance of Plant Drug and Fingerprints ofCoptis Chinensis

目的建立黄连的专属性对照物质及其核磁共振氢谱(HNMR)和高效液相色谱(HPLC)指纹图谱,用于其基源鉴定。方法采用规范化的程序获取黄连专属性对照物质并测定其1HNMR和HPLC指纹图谱;通过硅胶柱色谱法分离其化学成分,鉴定各单体化合物的结构,实现指纹图谱的解析。结果不同来源的黄连样品,其1HNMR和HPLC指纹图谱都有很好的重现性和特征性;从其专属性对照物质中分得盐酸巴马亭、盐酸小檗碱、盐酸表小檗碱、盐酸黄连碱和盐酸药根碱5个主要成分,经光谱分析鉴定了结构。黄连专属性对照物质的1HNMR和HPLC指纹图谱主要显示所分得小檗碱型生物碱的特征信号。结论黄连专属性对照物质的1HNMR和HPLC指纹图谱反映了其主要活性成分的结构和整体组成,适用于其基源鉴定和质量评价。

Objective To establish the control substance of plant drugCSPDof Coptis chinensis Franch. and its proton nuclear magnetic resonance1H NMRand high performance liquid chromatographyHPLCfingerprints for the purpose of original identification. Methods The CSPD and their 1H NMR and HPLC fingerprints of Coptis chinensis were obtained by standardized procedure. Chemical components were isolated from the CSPD by silica gel column chromatography. By elucidation of their structures the assignments of the characteristic signals in fingerprints could be achieved. Results The 1H NMR and HPLC fingerprints of the samples from various sources had wonderful reproducibility and characteristic features. Furthermore five main compounds were isolated from CSPD and their structures were authen-ticated by spectral analysis as palmatine chloride berberine chloride epiberberine chloride coptisine chloride and jatrorrhizine chloride respectively. The 1H NMR and HPLC fingerprints of the CSPD of Coptis chinensis showed mainly the characteristic signals of the berberine-type compounds isolated in this work. Conclusion The 1H NMR and HPLC fingerprints of the CSPD of Coptis chinensis exhibit the structures and total composition of the main active constituents in it and can be used for its original identification and quality evaluation.