二乙烯三胺戊乙酸(^(99)Tc-DTPA)测定肝硬化大鼠小肠通透性及其临床意义

Determination of intestinal permeability with (^(99)Tc-DTPA) in cirrhotic rats

目的 应用二乙烯三胺戊乙酸 (99Tc-DTPA )测定肝硬化大鼠小肠通透性 ,并探讨其影响因素。方法 肝硬化模型采用CCl4皮下诱导。小肠通透性测定应用 5 μCi99Tc-DTPA溶于 1ml水中灌胃并收集 5h及 2 4h尿待测。通过测定尿排出的99Tc-DTPA比率来反映肠粘膜对DTPA的通透状态 ,尿中排出越多反映肠道的通透性越大。结果 肝硬化腹水大鼠的 5h及 2 4h99Tc-DTPA尿排出率分别为 (8.43± 0 .91) %及 (16.3± 8.9% ) ,明显高于正常大鼠的排出率 ,也高于肝纤维化大鼠的DTPA排出率。吗叮啉组的 5h尿DTPA的排出率高于阿托品组 (P <0 .0 1) ,而 12h尿两组则无差异。结论 肝硬化大鼠99Tc-DTPA尿排出率明显高于正常对照组 ,而肝纤维化大鼠的排出率则与正常组大鼠无差异 ,提示只有严重肝病时小肠通透性才有改变 ,同时也提示 2

Objective To study changes of intestinal permeability with (()^(99m-)Tc-DTPA) (diethylenetriamine penta-acetic acid) in cirrhotic rats and its affecting factors.Methods Cirrhotic rats were induced by injection of 50% CCl_4 solution subcutaneously for 12 weeks. Intestinal permeability was evaluated by urinary excretion of (()^(99)Tc-DTPA) at 5h and 24h after intragastric gavage of 1ml 5μCi(()^(99)Tc-DTPA) solution. The greater the DTPA in urine, the greater the intestinal permeability.Results Urinary excretion of (()^(99)Tc-DTPA) of cirrhotic rats at 5h and 24h were (8.43)(±0.91%) and (0.63)(±8.9%) respectively, which were higher than that of normal and fibrotic rats. Urinary excretion of (()^(99)Tc-DTPA) in doperidone-treated rats at 5h was higher than that in atropine-treated rats, but no difference was found between two groups at 12h.Conclusion Urinary excretions of (()^(99)Tc-DTPA) of cirrhotic rats but not fibrotic rats were increased compared with normal rats, which suggested that intestinal permeability was increased only in severe liver diseases. 24h urinary excretion of (()^(99)Tc-DTPA) was not influenced by gastric emptying and intestinal transit pattern.