电针对慢性应激模型大鼠行为学及HPA轴相关激素的影响

Effect of Electroacupuncture on Changes of Behavior and Some Relat ed Hormones of Hypothalamus-pituitary-adrenal Axis in Chronic Stress Model Rats

目的 :探讨电针对慢性应激模型大鼠行为学的影响和对下丘脑 垂体 肾上腺轴 (HPA轴 )的调节作用。方法 :采用 7种不同的应激方式交替作用于SD大鼠 2 1d ,制成慢性应激模型 ,用韩氏电针仪 ,以 2Hz频率 ,0 .6mA电流强度 ,电针“百会”和“印堂”穴 ,每天 1次 ,每次 2 0min ,共2 1d。通过开野实验及糖水实验进行大鼠行为学检测 ,运用放射免疫法 (RIA)检测下丘脑CRF、垂体ACTH、肾上腺CORT的含量 ,并与空白组、模型组、百优解组及束缚组做比较。结果 :与空白组相比 ,模型组及模型束缚组开野实验的水平运动次数和垂直运动次数明显减少 ,糖水的摄入量明显减少 ,垂体ACTH、肾上腺CORT的含量明显升高 ,而电针组和百优解组可明显增加开野实验的垂直运动次数及糖水的摄入量 ,并可降低垂体ACTH、肾上腺CORT的分泌。结论 :慢性应激引起大鼠的活动性降低 ,探究行为减少和HPA轴亢进 ,电针可能通过调整垂体ACTH、肾上腺CORT的过度分泌 ,从而纠正HPA轴亢进 ,进而改善慢性应激大鼠的行为学表现。

Objective: To investigate the effect of electroacu puncture (EA) on changes of be havior and some related hormones of the hypothalamus-pituitary-adrenal (HPA) axi s in chronic stress rats. Methods: Fifty male SD Rats were eve nly randomized int o control, stress-model, Prozac-20, trussing-model and EA groups. Chronic s tres s model was established by using mixed stimulation including cool water swimming , tail-clamping, fasting, electric shock,etc., and trussing-model establishe d b y fastening the chronic stress rat to the animal table for 20 min, once daily, 21 days altogether. EA (2 Hz, 0.6 mA, 20 min) was applied to “Baihui” (GV 20) and “ Yintang”(EX-HN 3), once daily, continuously for 21 days. Prozac-20 (1.8 mg/k g) wa s given to the rats by using intragastric perfusion method. The rat’s behavior was observed by using Open-field method and the rat’s sucrose intake volume p er day under water-banning condition was calculated. The contents of corticotrop in releasing factor (CRF) in the hypothalamic tissue, adrenocorticotropic hormone (ACTH) in the pituitary and corticosterone (CORT) in the adrenal tissue were det ected with radioimmunoassay techniques. Results: Compared with control group, th e rats’ horizontal and vertical movement times and sucrose intake volume decrea s ed significantly while pituitary ACTH and adrenal CORT contents increased remark ably in both model and trussing-model groups (P<0.05). In compa rison with contro l group, the rats’ vertical movement times and sucrose intake volume increased c onsiderably while ACTH and CORT contents lowered significantly in both EA and P rozac-20 groups (P<0.05). Conclusion:Chronic stress may cause the stress rats to move less and result in hyperactivity of the HPA axis, whereas electroacupun cture can promote recovery of the rats’ abnormal behavior and adjust the secret ion of ACTH and CORT by inhibiting the hyperactivity of the HPA axis.