转人小肠三叶因子侧耳中hITF的表达及在大鼠体内生物活性研究

Expression of Human Intestinal Trefoil Factor (hITF) in Pleurotus ostreatus and Its Biological Activity Analysis In vivo

将人小肠三叶因子(hITF)用原生质体法导入糙皮侧耳(Pleurotusostreatus)中,检测表明,hITF在侧耳新鲜菌丝体中的表达量约为 10 0 0～ 2 0 0 0ng/g,最高约达 2 2 5 0ng/g.体内生物学活性研究证实,口服转hITF侧耳可有效地防止乙醇诱导的大鼠胃溃疡。

Human intestinal trefoilfactor (hITF) cDNA has been transferred into protoplasts of Pleurotus ostreatus by electroporation. Integration of the hITF cDNA into the genome Pleurotus ostreatus was confirmed by PCR analysis. Then competitive enzyme linked immunosorbent assay (ELISA) was developed for detection and quantitative analysis of human intestinal trefoil factor. Results show that the highest hITF protein content in mycelia is 2 000 similar to 2 250 ng/g, about 1.5% of the total soluble protein. The transgenic Pleurotus ostreatus is proved to have good biological activity of preventing rats from the gastric ulcer induced by ethanol, in which both of polysaccharides and hITF have a direct effect on ulcer tissue.