鼠mPC-1基因的克隆与特性分析

Cloning and Characterization of a Novel Gene: Mouse mPC-1

为深入研究人前列腺癌相关基因PC 1的生物功能和进化保守状况,从小鼠肾脏中克隆了全长cDNA序列,命名为mPC 1(GenBankAccNo.AY0 4 885 2 ).mPC 1基因cDNA全长为 2 193bp,主要定位于小鼠染色体 3A1 A2区域.mPC 1基因最大开放阅读框编码的蛋白质由 2 2 4个氨基酸组成,与人PC 1蛋白编码区存在 82 %的序列一致性,含有coiled coil结构域和PEST结构域.生物信息学分析表明,由 6个外显子组成的mPC 1基因与mD5 2高度同源,其中,第一外显子代表该基因的特异性序列,实验证据显示mPC 1基因具有自己的启动子,推测mPC 1与小鼠mD5 2可能是重叠基因.对小鼠 2 0种组织器官和不同发育阶段的胚胎组织cDNA的RT PCR检测证实,该基因主要在前列腺、肾和眼组织中表达,在胃和平滑肌中有少量表达,在其他组织中表达很弱或不表达.而mD5 2基因则几乎广泛存在于小鼠的各个组织器官中,因此,两个基因虽然序列上高度重叠却是独立调控的.综上所述,mPC 1基因可能是一个与人PC

In order to study the biological function and conservation of homology to the human prostate and colon cancer-associated gene (hPC-1), the complete cDNA sequence from mouse kidney was cloned. The gene was named mPC-1 (GenBank ACC No. AY048852), and its cDNA was 2 193 base pairs. The whole cDNA locus was mapped to mouse chromosome 3A1 similar to A2 by BLAST analysis to the mouse genome. The longest ORF of MPC-1 encoded the putative protein of 224 amino acids, which is 82% identical to hPC-1 coding region, with a coiled-coil domain and PEST domain in each. Bioinformatics analyses show that mPC-1 is comprised of six exons and highly homologous to mD52 and its first exon is a highly special gene sequence thereof. Special promoter of mPC-1 has been verified experimentally, therefore, it was assumed that mPC-1 is a gene overlapping with mouse mD52. RT-PCR performed in different mouse tissues or organs and various embryogenesis showed that the expression of the gene was strongly in prostate, kidney and eye, slightly low in stomach and smooth muscle, but not or very weakly in other tissues, however, the mD52 gene almost expressed ubiquitously in mouse tissues or organs. Consequently, the gene sequence between mPC-1 and mD52 is markedly overlapping in large-scale but modulated independently. Taken together, the results suggest that mPC-1 is a novel gene in coincidence to hPC-1 either structure or function.