摘要
探讨驱虫斑鸠菊(VW)注射液治疗白癜风的作用机制。进行以下实验探讨VW对小鼠免疫功能的影响:淋巴细胞转化试验测定小鼠脾T、B细胞增殖活性;脾细胞介导羊红细胞定量溶血分光光度法测定B细胞生成抗体活性,流式细胞法测定B细胞上CD19表达活性;迟发型超敏反应(DTH)试验测定T细胞活性、眼3H演-TdR掺入法测定T细胞分泌IL-2活性等。用酶学方法研究VW对小鼠体内酪氨酸酶的作用。用半定量逆转录聚合酶链反应技术检测酪氨酸酶基因表达活性。结果表明,VW可以明显抑制小鼠体内T、B细胞的增殖反应(P<0.01);对绵羊红细胞(SRBC)诱导的正常小鼠脾脏抗体形成细胞活性、CD19B细胞亚类表达、小鼠DTH反应和T细胞分泌IL-2活性也具有明显的抑制作用,这些抑制作用与药物浓度有一定的剂量效应关系。VW还可以提高小鼠血清酪氨酸酶活性,增强酪氨酸酶基因的表达。以上结果说明,VW可抑制小鼠免疫功能,可以从转录水平增强酪氨酸酶活性,进而促进黑素合成。
To explore the effect of the Vernoniaanthelmintica willd(VW) Injection on the immune function and tyrosinase activity in mice. The effect of VW Injection on spleen T?B cell proliferation and spleen T lymphocyte secretion of IL-2 activity were determined by -TdR incorperation method. Antibody activity were determined antibody forming cell assays, B lymphocyte surface antigen(CD19) were measured by flow cytometry. Zymologic method was used to evaluate tyrosinase activity. The level of tyrosinase mRNA were measured by RT-PCR technique. VW Injection could inhibit splenocyte proliferation and the antibody forming cell activity and decrease delayed type of hyper-sensitivity, while enhance the synthesis of tyrosinase of normal mice in vivo and the expression of tyrosinase mRNA in A375 human melanoma cell in vitro. VW Injection could inhibit the immune function and enhance the activity of tyrosinase.
出处
《生物技术通讯》
CAS
2004年第6期573-576,共4页
Letters in Biotechnology