H1oo在哺乳动物胚胎发育及其基因组重编程中的作用

Roles of Oocyte-specific Linker Histone during Mammalian Embryogenesis and Genomic Reprogramming

染色质结构可由转录抑制状态转变为转录激活状态,从而调节早期胚胎由母型基因控制转变为合子型基因控制。作为一种特殊类型的连接组蛋白——哺乳动物特异性连接组蛋白H1oo,其表达方式具有一定的时序性,但又与其他7种连接组蛋白亚型有所不同,H1oo不但能够在卵母细胞-胚胎发育转换过程中发挥功能,而且还可能在基因组重编程过程中起到关键性作用。分析研究卵母细胞特异性连接组蛋白,有助于认识染色质重构建、基因组重编程过程以及核移植的分子机制,而且可能对克隆效率的提高有所补益。

The changes of higher-order chromatin structure from transcriptional inactivation state to activation state influence the regulation of gene expression in early embryos which transit from maternal gene activity (MGA) to zygotic gene activity (ZGA). Oocyte-specific linker histone in mammalians as a special histone whose sequential expression patterns different from the other seven linker histone subtypes, not only takes part in the oocytic-embryonic switch during mammalian embryonenesis, also plays a critical role in genomic reprogramming. Through investigating oocyte-specific linker histone, the course of chromatin reorganization and genomic reprogramming, and the molecular mechanism of nuclear transfer will be well comprehended, which maybe make for the improvement of cloning efficiency.