大麦与白粉病菌互作中钙调素的细胞化学定位

Immuno-gold Localization of Calmodulin Interaction between Barley and Its Powdery Mildew Pathogen, Blumeria graminis f. sp. hoedei

对不同抗病性的大麦近等基因系受白粉病菌侵染诱导后的钙调素(CaM)变化进行了免疫细胞化学研究。结果表明,钙调素普遍存在于各种不同类型的细胞中,代谢活跃的细胞中CaM 标记密度相对较高;CaM分布于细胞核、细胞壁、叶绿体等细胞器中,其中细胞核标记密度最高。在健康叶片中,感病品系Ingrid伴胞中CaM的标记密度明显高于抗病品系mlo-3,而其他细胞中CaM标记密度没有明显差别。病原菌接种后,各种细胞的CaM标记密度均呈现一定程度的上升, 但:Ingrid上升幅度相对较大。在叶肉细胞中,mlo-3细胞核中CaM增加最明显。并对叶肉细胞和伴胞中CaM的变化进行了讨论。

The immuno-gold localization of calmodulin (CaM) in near isogenic line barley, Ingrid (Mlo) and BC Ingrid mlo-3 (mlo-3), induced by powdery mildew attack, was examined. CaM was detectable in all leaf cells and abundant CaM was found in the actively metabolic cell, such as companion cells. CaM existed in nuclei, cell wall, and chloroplast, especially in nuclei. In healthy leaves, the density of labeling for CaM in different cells in Ingrid and mlo-3 were similar except companion cells, in which CaM was much more in Ingrid than in mlo-3. The CaM was increased after inoculation with powdery mildew in other cells and organelles except that in the mesophyll nuclei. The changes of CaM in companion cells and mesophyll cells were also discussed.