苹果单倍体原生质体培养再生植株

PLANT REGENERATION FROM PROTOPLASTS OF HAPLOID INDUCED BY IRRADIATION IN APPLE

以富士单倍体试管苗幼叶和幼叶诱导的愈伤组织为材料制备原生质体 ,采用不同培养方法获得原生质体愈伤组织 ,在分化培养基上成功地诱导原生质体来源的愈伤组织再生不定芽。试验结果显示试管苗幼叶诱导的愈伤组织是制备高质量原生质体的良好材料 ,每g愈伤组织可制备原生质体 4 3× 1 0 6个 ;海藻酸钠球培养法是苹果原生质体培养的适宜方法 ,最终植板率达 1 5 % ;在分化培养基中添加精氨酸能有效提高愈伤组织不定芽分化能力 ,原生质体来源的愈伤组织分化频率高达 8 8%。

Protoplasts were prepared from young leaves and callus derived from young leaves of Fuji haploid shoots in vitro. Protoplast callus was recovered under different culture protocols and bud regeneration was accomplished from the protoplast callus. The results suggested that callus derived from the young leaves was suitable materials for preparation of protoplasts and 4.3×106 protoplasts were obtained from 1 g callus. Among 3 different culture protocols, protoplasts embedded in sodium alginate bead have the highest FPE of 1.5%, regeneration rate was up to 8.8%. The regeneration rate was significantly increased when Arg was added in the differentiation medium.