NTG诱导及核辐射改良稗草生防潜力菌研究

IMPROVEMENT OF BARNYARDGRASS POTENTIAL BIOCONTROL FUNGUS BY NTG INDUCING AND^(60)Co γ-RAY IRRADIATION

用亚硝基胍 (nitrosoguanidine ,NTG)诱变稗草生防潜力菌禾长蠕孢稗草专化型菌株(HGE) ,获得诱变菌株I2 62 ,其孢子产量比原始菌株 (HGE)提高 5 2 6%。以I2 62 为出发菌株 ,用60 Coγ射线 65 0Gy辐照 5 7min ,有 7 75 %的菌株产孢量高于出发菌株高 ,其中辐照菌株FⅡ12 1、FⅡ116和FⅡ14 0 的产孢量分别比I2 62 提高 5 4 4%、5 1 5 %和 41 7%。化学和物理技术相结合处理稗草生防潜力菌 ,获得的突变菌株产孢量比原始菌株提高 1倍以上。但高产孢突变菌株对稗草的致病性、防效与原始菌株相当。

Helminthosporium gramineum Rabenh f.sp. echinochloae (HGE), the Barnyardgrass (Echinochloa spp.) potential biocontrol fungus was induced by nitrosoguanidine(NTG,C 2H 5N 5O 3). The high yield of conidia production of inducing strain I 262 was obtained. Conidia production of I 262 increased 52.6% compared with its original fungus HGE. The experiments of nuclear irradiation were conducted by using I 262 as starting strain. I 262 was irradiated for 57 minutes by 60Co γ-rays at the dose of 650Gy. Among selecting mutants, there are 7.75% strains' conidia production higher than that of I 262. Among them, conidia production of mutants FⅡ 121、FⅡ 116 and FⅡ 140 increased 54.4%,51.5% and 41.7% compared with that of I 262, respectively. Conidia yield of mutants FⅡ 121 and FⅡ 116 were doubled compared with their original fungus HGE when using chemical in combine with physical technologies to treat the barnyardgrass' pathogen. The pathogenicity and control efficacy to barnyardgrass of high yield conidia production mutants were as the same as their original fungus HGE.