实验性肺癌尿激酶型纤溶酶原激活物和抑制物的表达及意义

SIGNIFICANCE AND EXPRESSION OF UROKINASE TYPE PLASMINOGEN ACTIVATOR AND PLASMINOGEN ACTIVATOR INHIBITOR IN EXPERIMENTAL LUNG CANCER

目的研究尿激酶型纤溶酶原激活物 (urokinasetypeplasminogenactivator,uPA)和抑制物 1(PAinhibitor,PAI 1)在实验性肺癌的发生、发展及侵袭转移过程中的作用。方法将Lewis肺癌瘤株腹腔注射C5 7/BL小鼠 ,于接种后 9、17、2 3天各处死 6只 ,取瘤、肺组织匀浆 ,低温离心取上清液 ,采用酶联免疫吸附 (enzyme likedimmunosorbentassay ,ELISA)法检测血浆和组织中uPA和PAI 1的含量 ,按环磷酰胺 +刺五加组、环磷酰胺组、刺五加组、肿瘤对照组和正常对照组分别进行对比分析。结果肿瘤组uPA和PAI 1含量高于正常对照组 ;环磷酰胺 +刺五加组血浆、瘤和肺组织中uPA和pAI 1活性低于肿瘤组。结论检测实验性肺癌uPA和PAI 1含量可能对其侵袭转移。

ObjectiveTo investigate the expression of urokinase type plasminogen activator(uPA) and PA inhibitor-1(PAI-1) in occurrence, progression and invasion of experimental lung cancer. MethodsC57/BL mice were inoculated with Lewis lung cancer strain, on 9, 17, 23 day post-inoculation, respectively, six mice were killed and the lung, tumor were removed aseptically. The plasma and tissues of the mice were homogenized, frozen and cleared of cellular debris by low speed centrifugation. The levels of uPA and PAI-1 of plasma and tissue were detected by ELISA in Lewis lung cancer C57/BL mice. The differences were compared between five groups as acanthopanax senticous plus cyclosphamide group, cyclophosphamid group, tumor control group and normal control group. ResultsThe levels of uPA and PAI-1 from tumor control group were higher than those from normal control group. The levels of uPA and PAI-1 in plasma and tumor from acanthopanax senticous plus cyclosphamide group were lower than those from tumor control group. ConclusionDetection of uPA and PAI-1 levels in experimental lung cancer may have certain referential value in evaluation of cancer invasion, metastasis and the prognosis.