RAPD标记对喀斯特高海拔山区地方玉米自交系遗传多样性研究

Study on Genetic Diversity of KASITE High Elevation Mountain Area Location Maize Inbred Lines By RAPD Markers

本文利用RAPD分子标记技术研究了适应我国喀斯特高海拔山区地方玉米种质的自交系的遗传多样性,从23条引 物对我国喀斯特高海拔山区玉米种质的地方自交系对其进行杂种优势群划分。从90个随机引物中筛选出23个多态性 好的引物扩增材料,产生出152条多态带,多态性位点比率为86.1%,通过UPGMA聚类分析,将我国喀斯特高海拔山区玉 米地方自交系划分为6个类群。

RAPD markers technique has been used to study the diversity of KASITE high elevation mountainous area maize local inbred lines. The screening of the 90 random primers allowed the selection of 23 polymorphic primer used for the analysis. A total of 152 polymorphic bands were produced from 23 selected primers. Through clustering on RAPD markers by UPGMA (Unweighed Pair-Group Method Using Arithmetic Average) , The ratio of polymorphic sites reached 86.1%. Clustering analysis was conducted based on the genetic similarity coefficient. The maize local-inbreds lines were classified into six groups.