摘要
本文以辣椒质核互作雄性不育为对象,进行RAPD体系优化及其标记的研究,结果表明在20μl的反应体系中, dNTP、Mg2+、引物和模板DNA的浓度分别在0.3mmol、2.5～2.75mmol、0.2～0.4mmol和30～60ng同时添加1UTaq 酶,退火温度控制在37.8～40.7℃,能够获得理想的扩增结果。利用该体系进行随机引物筛选获得不育系单显性标记5 个,恢复系单显性标记7个,共显性标记2个。
RAPD optimization system and markers were studied on cytoplasmic male sterile lines in pepper.The results indicated that ideal amplified profiles were obtained with concentrations of dNTP,Mg 2+, primer, model DNA and Taq enzyme wnich were 0.3mmol, 2.5-2.75mmol, 0.2-0.4mmol, 30-60ng and 1U respectively in 20μl reaction system and annealing temperature was7.8-40.7℃ in basic temperature cycle system and we have obtained 5 single dominant markers of male sterile line ,7 single dominant markers of restore line and 2 codominant markers with the system which belonged to lytoplasmic nuclear interaction.
出处
《种子》
CSCD
北大核心
2005年第2期24-26,30,共4页
Seed
基金
安徽省教育厅项目(2004JK086)
安徽省科技厅项目(04023069)
