人参皂甙和三七皂甙对突触体^(45)Ca摄取和[~3H]MK-801结合位点的影响

Effects of ginsenosides(GS) and sachinosides(SCS) on ^(45)Ca uptake into synaptosome and [~3H]MK-801 binding sites

采用放射性同位素标记方法,研究了GS和SCS对小鼠大脑突触体^(45)Ca摄取和[~3H]MK-801结合位点的影响。结果表明,GS和SCS 1mg/ml能明显抑制突触体对^(45)Ca摄取;SCS作用显著大于GS,且具有相对较强的高钾刺激依赖性。GS与SCS不同,能明显影响[~3H]MK-801与位点结合。结合三种L-型钙通道阻滞剂的类似实验结果,提示药物的钙阻滞强弱与其对NMDA受体-离子复合物通道影响无关,象二氢吡啶类钙阻滞剂Nit一样,GS的脑缺血保护作用可能涉及兼具钙阻滞和影响NMDA受体-离子复合物通道功能。

Effects of GS and SCS on Ca uptake into synaptosome and [~3H]MK-801 binding sites were studied by means of radioactive isotopical methods. It was shown that both of GS and SCS at the concentration of 1mg/ml could obviously inhibit Ca uptake of synaptosome; the effect of SCS was stronger than that of GS and more dependent on high potassium stimulation. However, only GS significantly influenced [~3H]MK-801 bound with its binding sites. Compared with the results from three Ltype calcium channel blockers, the data above indicate that there may their influence on the function of NMDA receptor-ion channel complex. Like dihydropyridines Nit, the protective effect of GS against brain ischemia may be involved in both its calcium channel antagonism and inhibitlon of [~3H]MK-801 binding sites.