地黄煎剂抑制异丙肾上腺素诱发的缺血大鼠脑Ca^(2+),Mg^(2+)-ATP酶活力升高

Rehmanniae Suppresses the Elevated Activity of Rat Cerebral Ca^(2+), Mg^(2+)-ATPase Induced By Isoprenaline

正常大鼠脑组织Ca^(2+),Mg^(2+)-ATP酶活力为2.8±0.7 μmol/Pi mg protein·h。大鼠ip异丙肾上腺素5mg/kg 24h造成脑缺血,其Ca^(2+),Mg^(2+)-ATP酶活力升高至8.8±0.8 μmol/Pi mg protein·h。经地黄2或4g/kg po给药后,大鼠缺血脑Ca^(2+),Mg^(2+)-ATP酶活力分别降至6.8±0.8和5.8±0.7 μmol/Pj mg protein·h,表明地黄可防止脑组织缺血损伤和ATP耗竭,提示地黄中可能含有钙拮抗活性物质。

The activity of cerebral Ca^(2+), Mg^(2+)-ATPase in normal rat was 2.8±0.7μmol/Pi mg protein·h. The ischemic model of rat brain was made by ip isoprenaline 5mg/kg for 24h in rat. The activity of Ca^(2+), Mg^(2+)-ATPase of the ischemic cerebrum very significantly increased to 8.8±0.8μmol/Pi mg protein·h. Medica- tion of Radix Rehmanniae 2 or 4g/kg po for 6h in above ischemic rat, the activities of cerebral Ca^(2+), Mg^(2+)-ATPase were very significantly decreased to 6.8±0.8 and 5.8±0.7μmol/Pi mg protein·h respectively. The results showed that Radix Rehmanniae could prevent rat cerebrum from ischemic injury and ATP depletion. The results also suggested that Radix Rehmanniae could contain the active substance of Ca^(2+) antagonism or inhibitor of Ca^(2+), Mg^(2+)-ATPase.