定量聚合酶链反应检测血清乙型肝炎病毒DNA及其意义

Quantitative Polymerase Chain Reaction for Detection of HBV DNA and Clinical Significance in Hepatitis B

采用定量ＰＣＲ技术检测了１４０例病毒性肝炎患者及３１例ＨＢｓＡｇ携带者血清乙型肝炎病毒（ＨＢＶ）ＤＮＡ基因含量。结果显示１２３例乙型肝炎患者及３１例ＨＢｓＡｇ携带者血清ＨＢＶＤＮＡ基因含量范围在１０１至１０９拷贝／微升，１７例其它类型肝炎及３０例健康正常人均未检出ＨＢＶＤＮＡ；与定性ＰＣＲ及分子斑点杂交检测比较，定量ＰＣＲ的灵敏度更高。该方法是一种快速、敏感、特异的定量ＰＣＲ技术，可用于乙型肝炎的临床诊断和实验研究。

A quantitative PCR assay is described for serum HBV DNA which is based on Ampli Sensor Assay in 140 hepatitis patients and 31 HBsAg carriers.The result showed the amount of HBV DNA ranged from 10￣1to 10￣9 copies/μl in 123 hepatitis B and 31 HBsAg carriers, HBV DNA was negative in 17 non-B hepatitis and 30 normal controls. Its sensitivity of this method was higher than that of the qualitative PCR and dot - hybridization analysis. The quantitative PCR is a specific and sensitive method which may be used in routine detection and research of hepatitis B.