摘要
We evaluated the function of the fresh and cryopresereed newborn rat testicular tissue transplanted into outbred adult castrated male rats without immunosuppression for 30 days.Testis grafts were evaluated morphologically and histologically,and testosterone(T)levels of serum and homogenate of testis grafts were determined in recipients by 30 days after transplantation.The fresh tissues(GroupI)and cryopreserved tissues(Group Ⅱ),from the 2nd or 3rd day of postnatal,showed an overall increase in growth,with maintenance of architecture and minimal lymphocytic infiltration.Thirty days after implantation,mean(±s)serum T concentrations(0.986±0.028 nmol/L in group I;1.113±0.201 nmol/L in group Ⅱ)were significantly higher than that prior to transplantation(<0.347 nmol/L,respectively;P<0.01 and P<0.01).After transplantation,however,mean serum T levels between two groups were not significantly different(P<0.05),and their grafting homogenate T levels(0.944±0.164 nmol/g in group I;1.019±0.172 nmol/g in group Ⅱ) were also not significantly different between two groups(P>0.05).Our data suggest that the testicular tissue of newborn rats can not only survive,grow and differentiate,but also can produce enough T detectable in vivo after transplantation and no significant differences were found between fresh tissue and frozen tissue transplanted.
We evaluated the function of the fresh and cryopresereed newborn rat testicular tissue transplanted into outbred adult castrated male rats without immunosuppression for 30 days.Testis grafts were evaluated morphologically and histologically,and testosterone(T)levels of serum and homogenate of testis grafts were determined in recipients by 30 days after transplantation.The fresh tissues(GroupI)and cryopreserved tissues(Group Ⅱ),from the 2nd or 3rd day of postnatal,showed an overall increase in growth,with maintenance of architecture and minimal lymphocytic infiltration.Thirty days after implantation,mean(±s)serum T concentrations(0.986±0.028 nmol/L in group I;1.113±0.201 nmol/L in group Ⅱ)were significantly higher than that prior to transplantation(<0.347 nmol/L,respectively;P<0.01 and P<0.01).After transplantation,however,mean serum T levels between two groups were not significantly different(P<0.05),and their grafting homogenate T levels(0.944±0.164 nmol/g in group I;1.019±0.172 nmol/g in group Ⅱ) were also not significantly different between two groups(P>0.05).Our data suggest that the testicular tissue of newborn rats can not only survive,grow and differentiate,but also can produce enough T detectable in vivo after transplantation and no significant differences were found between fresh tissue and frozen tissue transplanted.
