转染IL-16基因对T淋巴瘤细胞的增殖抑制作用

Proliferation of T lymphoma cells was inhibited by transfecting IL-16 gene

目的 构建 IL-1 6真核表达载体 ,研究其对 T淋巴瘤细胞的增殖抑制作用。 方法 应用 RT-PCR技术从人外周血单个核细胞中获取 IL-1 6c DNA,构建并鉴定真核表达载体 Pc DNA3 -IL-1 6转染 Karpas T淋巴瘤细胞 ,采用 MTT法和流式细胞术分析 IL-1 6对 Karpas T淋巴瘤细胞的增殖抑制。 结果 PT-PCR显示转染 Pc DNA3 -IL-1 6的 Karpas T淋巴瘤细胞高表达 IL-1 6m RNA,MTT法检测显示其对 Karpas T淋巴瘤细胞具有抑制作用 ,流式细胞术检测转染 Pc DNA3 -IL-1 6的 Karpas T淋巴瘤细胞高表达 CD95分子。 结论 成功构建了真核表达载体 Pc DNA3 -IL-1 6,转染 Karpas T淋巴瘤细胞能使其生长受到明显抑制 ,并且 CD95分子表达增高 ,可能与诱导细胞凋亡有关 ,说明向肿瘤细胞转染 IL-1 6是一种有用的肿瘤生物治疗方法。

Objective To construct human IL 16 eukaryotic expression vector,and analyse its function of inhibiting proliferation of tumor cells. Methods The human IL 16 cDNA was amplified by RT PCR from peripheral blood mononuclear cells, and cloned into the PUC 18 T vector. Then the eukaryotic expression vector PcDNA3 IL 16 containing IL 16 cDNA was constructed and identified. The Karpas T lymphoma cells were transfected with PcDNA3 IL 16. To analyse its function of inhibiting proliferation by MTT and flow cytomery. Results The recombinant vector PcDNA3 IL 16 was transfected into Karpas T lymphoma cells. RT PCR indicated the IL 16 mRNA was highly expressed in the Karpas cells. Flow cytometry indicated the CD95 was higher expressed than control. The biological activity through MTT assay showed the proliferation of Karpas cells were sharply inhibited. Conclusion The eukaryotic expression vector PcDNA3 IL 16 containing IL 16 cDNA was successfully constructed. The proliferation of Karpas T lymphoma cells can be inhibited and the CD95 is highly expressed by transfecting PcDNA3 IL 16.These results suggest it is an useful method to transfect IL 16 gene into tumor cells for tumor biotherapy.