甘糖酯对培养的牛脑微血管平滑肌细胞增殖的影响

EFFECTS OF PROPYLENE GLYCOL MANNATE SUL-FATE(PGMS) ON THE PROLIFERATION OF BOVINE CEREBRAL MICROVESSEL SMOOTH MUSCLE CELLS IN CULTURE

应用牛脑微血管平滑肌细胞(BCMSMCs)体外培养技术,观察了酸性多糖药物甘糖酯(PGMS)对10％小牛血清(FCS)、白细胞介素1(IL-1)致BCMSMCs过度增殖的影响。将培养的5～8代细胞接种于96孔板,正常组加入含10％FCS的MEM培养液(或含有50u/ml IL-1的0.4％FCS培养液),给药组分别加入含有PGMS0.05、0.1、0.2、0.4、0.8、1.6mg/ml浓度的10％FCS培养液(或50u/ml IL-1的0.4％FCS培养液),以结晶紫染色,MTT比色,测定72h后细胞生长情况。结果显示,与10％FCS组和IL-1组比较,PGMS组BCMSMCs的增殖受到明显抑制(P<0.01),提示PGMS对10％FCS和IL-1引起的BCMSMCs异常增殖具有明显的抑制作用。

The abnormal proliferation of vascular smooth muscle cells after endothe-lial injury is postulated to be the main pathophysiological process in atherosclerosis (AS). The effects of propylene glycol mannate sulfate(PGMS) on the proliferation of bovine cerebral microvessel smooth muscle cslls (BCMSMCs) induced by 10% fetal calf serum (FCS) and interleukin l(IL-1) were investigated in culture. 5 - 8 stage subcultured BCMSMCs were incubated into 96-well dish- With either 10% FCS or IL-1 (50u/ml) to produce BCMSMCs proliferation , the inhibitory effects of PGMS on proliferation of BCMSMCs were investigated. The results shows that PGMS could inhibit the proliferation of quiescent BCMSMCs induced by 10% PCS. The growth of cells was inhibited, comparing with normal control 72hours after the serum addition as determined by crystal violet stainning and MTTmethod. The proliferation of quiescent BCMSMCs induced by IL-1 (50u/ml) was also inhibited by PGMS as determined by crystal violet stainning and MTT method. The results suggested that PGMS inhibit the proliferation of BCMSMCs induced by 10% FCS and IL-1 ,and the use of PGMS may probably play an important role in the treatment of cerebrovascular disease.