大豆耐盐离体筛选分化再生植株的初步研究

PRELIMANARY STUDIES ON THE SCREENING OF NaCI-TOLERANTE SOYBEAN PLANTLET REGENERATED FROM TISSUE CULTURE

大豆(Glycine max)小真叶外植体直接接种到含不同浓度NaCl的选择培养基中,愈伤组织诱导率随NaCl浓度增高而降低,获得再生植株的最高NaCl浓度为0.25％。用连续逐级转移愈伤组织不断加强选择压的方法,可使筛选耐盐再生植株的最高NaCl浓度提高到0.3％。

The explant of young true leaves of soybean were cultured in the media containing NaCl. The result showed that the rate of callus induction was decreased as the Nacl concentration was increasing. In the media of high NaCl concentration was in creasing. In the media of high NaCl concentration the pullulation rate of callus was slower, the volume smaller. However, the structure because closer, texture harder. Differentiation frequency of the regenerated plantlet was decreased as the NaCl concentration was increasing. The maximum NaCl concentration in which regenerated plantlet could be obtained was 0.25%. Comparing with the control regenerated plantlets, they had short plant, thick stem and thick leaf which seemed consistently to be with succulence of halophyte. The small number of callus obtained in media containing NaCl 0.3% only had differentiation of bud. But by transfer the callus tissues gradually from lower NaCl concentration media to higher concentration regenerated plantlet could differentiate out in the same selection media (NaCl 0.3%). The frequency of differentiation is 2.5%. Small number of callus in media containing more NaCl (0.35%) could differentiate bud as well. It indicates that the maximum NaCl concentration of screening NaCl-tolerante regenerated plantlet can increase to 0.3% then it is passible to reach to 0.35% by constantly enhancing selection pressure.