正常人和不育患者精子膜上凝集素受体的数量与部分性质

PROPERTIES AND QUANTITIES OF THE LECTIN RECEPTOR ON THE SURFACE OF NORMAL AND INFERTILITY SPERMS

应用(125)I-CKL对正常人和不育患者精子膜上凝集素受体进行研究的结果表明,不育精子膜上凝集素受体的差异,可能是不育的原因之一。

The binding rate saturation curve and Scatchard plot of the reaction between 125I-CKL and the normal/infertility sperms have been studied. The results show that the normal cells have the higher and wider optimun pH range than the infertility ones. Kd value and saturation quantity of the receptors of CKL on normal and infertility sperms are different, which are 41. 67μg/ mL and 2. 5μg/mL, 1. 3×106/cell and 7. 7× 106/cell,respectively. The concentration of the sperms has different effect on the binding abilities to the 126I-CKL. Monosacchride inhibitions give irregular competitive curve. The results show that the structure of the receptors are complicated, the chemistry properties of normal and infertility sperms are sharply different.