ICR小鼠人工感染泰泽氏菌的组织病变观察

Histopathologic manifestation in ICR mice experimentally infected with Clostridium Piliformis

20只ICR小鼠经尾静脉途径人工感染泰泽氏菌。感染小鼠健康活泼 ,与对照小鼠无区别。感染后 4～ 7天 ,10只经醋酸可的松激发的小鼠其肝脏均表现出轻重不等的散在、灶性病变以至弥漫性灶性坏死 ,Giemsa染色镜检可见数量不等、典型的泰泽氏菌 ,细菌培养阴性。 10只未经激发小鼠和 5只对照小鼠均未见有病变 ,镜检也未查到泰泽氏菌。上述试验结果表明 。

ICR mice were inoculated with Clostridium piliformis via tail vein. Clinical signs of Tyzzer's disease in infected mice were not observed. Gross lesion of liver with varying severity in 10 mice treated with cortisone acetate was observed 4 to 7 days post-infection. Typical Clostridium piliformis on stamp preparation of postmortem liver were seen in a “pick-up-stick” arrangement within or beyond hepatocytes with Giemsa stain. Bacterial culture on artificial media was negative. Meanwhile no gross lesions and Clostridium piliformis were identified both in 5 sham control mice and 10 no cortisone treatment mice, The above results suggest that inducing gross lesion of liver and Clostridium piliformis propagation are associated with immunological state of experimental animals