实验动物饲料微生物学质量的检测

Examination of the Microbiological Quality of the Feed for Laboratory Animals

为了探讨粉料、制粒后出机料、成品料的微生物学质量变化情况，指导实际工作，应用常规的检测方法，对普通级实验动物鼠及兔饲料细菌总数、霉菌总数、大肠埃希菌最近似数（MPN）及致病性沙门菌等进行了检测。其结果表明：1. 所有检测样品均未检出大肠埃希菌及致病性沙门菌。2. 所有粉料的细菌总数及霉菌总数均超过国标（鼠料5×104、1×102，兔料1×105、1×102）。3. 出机时的鼠料其微生物学质量均符合国家标准，而兔料的细菌总数及霉菌总数均超标。4. 成品鼠料，贮存一周，细菌总数及霉菌总数均低于国标，贮存二周，霉菌总数低于国标，但细菌总数大于国标，三周、四周后细菌总数及霉菌总数均大于国标；成品兔料，其细菌数和霉菌数均高于国标。提示：出机时的鼠料可直接饲喂一级动物，成品鼠料，保存时间不易超过一周；兔料必须经过80 ℃、2 h 或60Co照射等处理方可食用。

The microbiological quality of the feed for laboratory animals was examined by routine methods.All the feed examined was not contaminated by Salmonella and Escherichia. The total number of bacteria and mould of powder feed overran GB (mouse feed was 5×104,1×102 and rabbit feed was 1×105,1×102 respectivily).The microbiological quality of mouse feed coming from machine conformed to GB until its storage for a week, but the rabbit feed did not. These results suggest that mouse grain feed be not keep more than one week and rabbit grain feed be pretreated before use, say at 80 ℃ for 2 hours or with 60 Co irradiation..