人CD55基因的克隆及其转基因构件的组装

Clonig of Human CD55 Gene and Recombing Its Transgenic Constructs

本实验采用人肝组织作为RNA的来源 ,经RT -PCR扩增得到CD55基因的cDNA片段。与人α -珠蛋白启动子及其polyA序列重组 ,插入质粒载体pGEM - 5zf,获得了可用于受精卵原核显微注射的基因构件 ,为建立人CD55转基因动物模型奠定了基础。

First,we use human hepatic RNA as template and got CD55 cDNA by PT-PCR. Secondly, CD55 cDNA is combined with promoter and polyA of human α-globin, then inserted into plasmid pGEM-5zf. At last,we got a transgenic constructs' of pGEM-5zf-α-globin-CD55 cDNA'. The constructs may use to found human CD55 transgenic animal model.