HLA-Ⅱ类基因DR_α及DRB_1 0401转基因小鼠模型的遗传与繁殖

Breeding and Genetic of HLA-DRα,DRB_1 0401 Transgenic Mice Model

采用类系祖建系的方法 ,对HLA -Ⅱ类基因DRα 和DRB1 0 4 0 1转基因小鼠模型进行了繁育。通过PCR和Southernblot分子杂交的方法 ,检测小鼠尾组织的DNA样品 ,以确定DR基因的整合和复制情况。结果表明 ,HLA -Ⅱ类基因DR已稳定地遗传至第五代(F5) ;共产仔 32 6只 ,PCR检测出阳性小鼠 95只 ,阳性率为 2 9.1 %。PCR—Southern印迹杂交检测 ,发现 68只仔鼠整合有DR基因 ,总有效率为 2 0 9%。经Nothern杂交和RT -PCR检测HLA -DR基因在脾脏和肾脏中均有表达。

s We have constructed transgenic mice Model on DRα and DRB 1 *0401 of MHC-II molecules by microinjected techniques on germ nucleus of zygotes, DRα and DRB 10401 genes were microinjected into the fertilized eggs of C57BL/6×DBA/1 hybrid mice and transplanted into the oviducts of pseudopregnancy female mice. The total integration rate and efficient rate of transgene were 29.1% and 20.9% assessed by PCR, Southern blot, Northern blot and RT-PCR analysis.There were 5 founders of all injected mice, which had steadily inherited to the fifth generation. It was found that 95 mice were positive by PCR and 68 mice were integrated exogenouse gene by Southern blot analysis DR α and DRB 1 *0401 gene were experssed on spleen and kidney of transgenic mice. This experiment on the breeding and genetic of DRα and DRB 1 *0401 transgenic model is a success.