阿尔茨海默症转基因动物模型脑组织病理学及免疫组化研究

Pathomorphological and Immunohistochemical Findings in Brains of APP Transgenic Model

日的通过组织病理学和免疫组织化学方法，验证阿尔茨海默症转基因动物模型。方法取转基因小鼠脑组织，冠状切面中１／３部位，脱水、包埋，进行组织病理学观察，并对相关抗体进行免疫组织化学研究。结果免疫组化显示在大脑皮层、小脑及海马的神经细胞有Ａβ沉淀形成。ＡＰＰ转基因鼠早老素的阳性细胞数及表达量多于对照鼠。刚果红染色可见大脑皮层间有淀粉样物质形成。结论从病理学角度验证此模型的表型与人类病变的相似性，并证明早老素－１可加速淀粉样沉淀的形成，二者的作用是相互的。

Objective To study the neuropathological features of transgenic model for Alzheimer's disease by pathomorphological and immunohistochemical methods. Methods Tissue mass was taken from the transgenic mice's brain for dehydration and embedding, congo red staining and immunohistochemical staining. Results Immunohistochemistry revealed plaques in pallium, cerebellum and hippocampus. The expression of PS-1 in transgenic mice obviously increased comparing with control. Congo red staining showed amyloid deposition in intercellular. Conclusion The transgenic model's pathomorphological display conform to the Alzhimer's disease and it could be used for further study.