巴马小型猪群体遗传结构的随机扩增多态DNA分析

Analysis of Genetic Constrution of Population in Bama Miniature Pigs by RAPD

目的分析巴马小型猪的群体遗传结构。方法应用经过筛选的 ３１条引物对巴马小型猪个体基因组ＤＮＡ进行ＲＡＰＤ扩增，利用从ｉｎｔｅｒｎｅｔ网上下载的软件ＲＡＰＤ ｉｓｔａｎｃｅ Ｐａｃｋａｇｅ ｖｅｒ－ｓｉｏｎ１．０４软件对实验结果进行分析，计算不同个体间的相似系数。结果经ＲＡＰＤ反应，共扩增出２７５条带，其中多态性带８５条，多态性带频率在０～６６．７％之间，平均为２７．７％。不同个体猪拥有的ＲＡＰＤ条带具有差异，但拥有相同条带个体猪比率较高。该群体猪的相似系数（Ｆ）为０．９２８（０．７８～０．９７），平均等位基因频率（ｑ）为０．７３２，最低平均杂合率（Ｈ）为０．２８６。结论巴马小型猪个体具有较好的遗传一致性和遗传稳定性。

Objective To analyse the genetic construction of population in Bama miniature pigs. Methods 31 selected single primers were used to amplify genomic DNA of Bama miniature pigs. The amplified fragments were analysed by the RAPD istance package version 1.04 and the similarity index was calculated. Results 275 bands were obtained in which polymorphisic bands were 85, the frenquency of polymorphisic bands were between 0-66.7%. Different individual pig has various bands, but the frenquency of common band was higher. Based on RAPD patters similarity index (F) calculated was 0.928 (0.78-0.96), mean allelic frequency (q) was 0. 732, and minimun heterozygosites (H) was 0.268. Conclusion Bama,miniature pigs reached a higher degree of homogeneity than ordinary bred pigs.