鼠脑缺血后神经元凋亡与P53的关系及碱性成纤维细胞生长因子的影响

The relationship between apoptosis of neurons and P53 gene and the effect of basic fibroblast growth factor on them following cerebral ischemia

目的 研究脑缺血再灌注后P5 3与细胞凋亡的关系和外源性碱性成纤维细胞生长因子 (bFGF)的影响。方法 用原位杂交、免疫组化及原位末端标记的方法观察大鼠大脑中动脉闭塞 2h后再通即刻至 72h脑组织P5 3基因的表达与凋亡细胞的分布及侧脑室注射外源性bFGF对它们的影响。结果 缺血 2h及再灌注即刻可见P5 3的表达和凋亡细胞 ,P5 3mRNA及其蛋白的表达高峰在缺血再灌注后 2 4h ,凋亡细胞数高峰在再灌注 2 4～ 48h。bFGF组与缺血组相比 ,6～ 48h各时间点P5 3表达减弱 ,凋亡细胞数明显减少(P <0 .0 5 ,P<0 .0 1)。结论 脑缺血再灌注后诱导P5 3的表达和细胞凋亡 ;外源性bFGF能抑制脑缺血再灌注后P5

Objective To study the relationship between cell apoptosis and the expression of P53 gene as well as the effect of exogenous bFGF on them following cerebral ischemia reperfusion.Methods P53mRNA and protein expression as well as the effect of exogenous bFGF on them were examined using in situ hybridization and immunohistochemistry at 0～72 h of reperfusion after occlusion of middle cerebral artery (MCA) for 2 h in rats.Simultaneously, the distribution of cell apoptosis was observed.Results The expression of P53 gene reached peak at 24 h and cell apoptosis reached peak at 24～48 h of reperfusion.Both of them decreased in bFGF treated rats as compared with ischemic rats (P<0.01).Conclusions Cerebral ischemia reperfusion can induce P53 gene expression and apoptosis of cells.Exogenous bFGF can suppress the expression of P53 and cell apoptosis after cerebral ischemia reperfusion.