17β-雌二醇和氧化型低密度脂蛋白对内皮细胞产生炎性细胞因子的影响

Effects of 17β-estradiol and oxidized low-density lipoproteins on cultured endothelial cells to produce inflammatory cytokines

目的 研究氧化型低密度脂蛋白 (ox LDL)对内皮细胞产生促炎性细胞因子白细胞介素 6 (IL 6 )和肿瘤坏死因子 (TNF)的影响及 17β 雌二醇的作用。 方法 以体外培养的人脐静脉内皮细胞 (HUVEC)为模型 ,将ox LDL与HUVEC孵育及HUVEC经它莫西芬 (tamoxifen)和 (或 ) 17β 雌二醇预处理后 ,再与ox LDL孵育 ,检测培养液中IL 6和TNF的活性。结果 ox LDL可明显增加内皮细胞产生IL 6和TNF。ox LDL 2 0mg/L时 ,TNF为 (0 .72± 0 .0 8) μg/L ,IL 6为 (92 .2 8± 6 .2 9)U/ml;经 17β 雌二醇预处理后 ,细胞在ox LDL作用后 ,IL 6和TNF活性与对照组比较 ,差异无显著性意义 ;它莫西芬和 17β 雌二醇处理内皮细胞后 ,再加ox LDL ,结果显示IL 6和TNF活性增高 ,与对照组比较 ,差异有显著性意义。结论 ox LDL可促进血管内皮细胞产生促炎性细胞因子IL 6和TNF。雌激素可明显抑制ox LDL引起的内皮细胞产生IL 6和TNF ,此作用可被它莫西芬拮抗。

Objective To study the effects of 17β-estradiol on the production of interleukin-6 (IL-6) and tumor necrosis factor (TNF) induced by oxidized low-density lipoproteins (ox-LDL) in vascular endothelial cells (VECs).Methods The levels of IL-6 and TNF were measured in cultured human umbilical vein endothelial cells.Results ox-LDL significantly increased the activity of IL-6 and TNF in cultured endothelial cells.With ox-LDL 20 mg/L,TNF activity was (0.72±0.08)μg/L and IL-6 activity was (92.28±6.29)U/ml,P< 0.01. 17β-estradiol could significantly decrease the production of IL-6 and TNF induced by ox-LDL in cultured endothelial cells.However,when tamoxifen and 17β-estradiol were added,the levels of IL-6 and TNF significantly increased as compared with those in the control group (P>0.05).Conclusions ox-LDL can obviously promote the product of IL-6 and TNF in vascular endothelial cells.17β-estradiol can significantly inhibit the production of IL-6 and TNF in endothelial cells induced by ox-LDL,but tamoxifen is able to block this effect.