人血浆第Ⅴ因子蛋白含量的免疫测定

A NOVEL ENZYME IMMUNOASSAY FOR DETERMINATION OF HUMAN PLASMA FACTOR V

目的建立一种双抗体夹心 ELISA,以测定人血浆第 V 因子(FV)抗原含量。方法采用兔抗人 FV 抗体为包被抗体,FV 单抗为检测抗体,首次对中国正常人(n=26)和一个遗传性 FV 缺乏家系的有关成员血浆中的 FV 抗原进行了检测。结果该法线性范围广、特异性强、灵敏度高、重复性较好,和 FV 活性测定法有很好的相关性;正常人血浆 FV 抗原星偏态分布:该家系纯合子血浆 FV 抗原量只有正常人的2%,证实该家系为Ⅰ型遗传性 FV 缺乏症。结论建立了一种新的 FV 蛋白定量测定法,该法可以对 FV 缺乏症进行辅助诊断和分型。

Ob jective To establish a novel quantitative method for determination of human plasmafactor V.method A double-antibody sandwich ELISA using the rabbit anti-human FV antibody for platecoating and monoclonal antibody against FV as second antibody was applied to quantify FV antigen in plasmasfrom normal Chinese adults(n=26)and members of a hereditary FV deficiency family.Results This methodwas specific and sensitive with a wide linear range.It was in excellent correlation with the coagulantactivity assay.The distribution of FV concentration in plasmas from 26 normal subjects was skewed.Ahomozygote for hereditary FV deficiency had 2% of normal plasma FV concentration,indicating the presenceof a type Ⅰ factor Ⅴ deficiency.Conclusion The FV quantitative assay was useful for the diagnosis andclassification of FV deficiency.