实验性青光眼视网膜神经节细胞p53基因表达的检测

Detection of the expression of p53 gene on retinal ganglion cells of rabbit eyes with intraocular hypertension

目的检测不同眼压状态下实验性青光眼视网膜神经节细胞（ＲＧＣｓ）ｐ５３基因的表达。方法家兔１６只，任选 １眼作实验，另眼对照。 ２％甲基纤维素０． １０～０． １５ ｍｌ前房注射制做高眼压模型，根据眼压值低、中、高不同对动物进行分组。分期取材、石蜡切片、免疫组化染色检测 ｐ５３蛋白的表达情况。采用计算机图像分析技术对 ＲＧＣｓ密度进行定量分析。结果凋亡率高低依次为：眼压较低组（１．８６％）＞眼压中等升高组（０．９３％）＞眼压较高组（０．５３％）＞对照组（０．０５％）（Ｐ＜０．０１）。结论与前人对ＰＯＡＧ患者的研究结果相反，在眼压急速升高的模型眼中，凋亡主要发生在实验的早期。

Objective To detect the expression on p53 gene in retinal ganglion cells(RGCs) of rabbit eyes with different degrees of intraocular hypertension. Methods intraocular pressure (IOP) was elevated by intracameral injection of 0. 10 0. 15 ml of 2% methylcellulose. Animals were grouped up by different values of IOP. The rabbit eyes were enucleated, fixed, paraffin-embedded and cut into serial 6- μm thin sections. For histological evaluation, the slides were stained with DAB chromogen (conducting an immuohistochemical experiment), and were examined by light microscopic and automatic image analysis. Results The number of RGCs with a positive reaction for P53 monoclonal antibody was statistically higher in the experimental eyes than in their control fellow eyes(P < 0. 01 ),and apoptosis was mainly found in the early stage of research. Conclusion Our research presents a new alternative view of apoptosis in experimental glaucoma(According to former reports,apoptosis was mainly found in the advanced stage of the patients with POAG).