β-葡糖醛酸酶在恒河猴组织中的分布

Distribution of β-Glucuronidase Activity in macaca mulatta Tissues

目的 阐明β -葡糖醛酸酶 (β-G)在正常恒河猴组织中的分布。方法 对实验恒河猴主要组织的冰冻切片采用后偶合技术进行了染色和显微观察。结果 肾上腺皮质束状带和网状带细胞 ,肝脏、睾丸、卵巢、胃肠道的实质细胞、枯否细胞、软骨细胞、卵巢间质腺细胞等富含β -G而深染蓝色。肾上腺皮质球状带细胞、血细胞、脑、肌肉、胆囊和胰腺的实质细胞等酶含量少而染色较弱。软骨基质 ,小脑皮质分子层和颗粒层等部位酶呈阴性。结论 动物的种系和品种不同 ,器官组织内

Objective To understand the histochemical distribution of β-glucuronidase activity in macaca mulatta tissues Methods Microscopic obeservation was conducted by using frozen section and postcoupling stain technique Results The enzyme-rich cells were the zona reticularis and zona fascicula cells of adrenal gland,parenchymal cells of testis,ovary,liver and gastrointestine, Kuffer’s cell,chondrocyte and interstitial gland cell of ovary The enzyme-poor cells were xona glomerulosa cells of adrenal gland,hemocyte,parenchymal cells of the brain,muscle,gallbladder and pancreas The enzyme was negative in the regions of cartilage martrix,molecular layer and granular layer of cerebellum Conclusion The different patterns of distribution of β-glucuronidase appeared in the tissues