颈动脉负压分流制作大鼠全脑缺血/再灌注模型

A Novel Model of Cerebral Ischemia/reperfusion Induced by Carotid Artery Negative Pressure Shunt in Rats

目的 根据解剖学和血流动力学原理建立新型大鼠全脑缺血 再灌注模型。方法 夹闭大鼠双侧颈总动脉 ,同时经右颈外动脉持续抽吸颈总动脉内血液 ,造成大鼠全脑缺血 ,抽出的血液从左股静脉回输 ;停止抽吸血液 ,去除微动脉夹 ,开始再灌注。应用脑电图、光镜和电镜等评定脑缺血的效果。结果 实验组大鼠脑电图、光镜和电镜检查均显示明显的缺血改变。结论 本模型具有全脑缺血效果可靠、再灌注充分、制备简便、成功率高并可经颈动脉注入药物等优点 ,适用于全脑缺血

Objective To establish a new model of whole cerebral ischemia/reperfusion in rats on basis of anatomic and hemodynamic principle Methods Whole cerebral ischemia in rats was induced by clamping the bilateral common carotid arteries and drawing some blood from right common carotid artery continuously through right external carotid artery Meanwhile the drawn blood was retransfused into left femoral vein Reperfusion began after stopping drawing blood and removing the artery clamps Electroencephalograph(EEG),light microscope(LM) and electron microscope(EM) were used to assess the result of ischemia Results Compared with control group,the whole cerebral in the experimental group showed obvious ischemia changes in EEG,LM and EM Conclusion This novel model has several advantages such as the reliable whole cerebral ischemia,sufficient reperfusion,high rate of success,fit for injecting reagent through carotid artery,and is useful to the study of whole cerebral ischemia/reperfusion