两个不同地区东方田鼠杂交子代RAPD标记分析

Genomic RAPD Analysis on Hybrid Field Voles(Microtus Fortis) from Two Regions in China

目的 研究不同地区东方田鼠杂交后产下的子代的遗传特性。方法 筛选 4条 1 0bp随机引物对宁夏和洞庭湖地区东方田鼠杂交子代的基因组进行随机扩增多态DNA(RAPD)分析 ,并对不同地区亲代以及子代相互之间的基因组DNA进行相似性分析。结果 ①所有东方田鼠均有相同的扩增片段出现 ;②两个不同地区的亲代分别有特异性片般 ;③亲代的DNA带型均能在子代中找到 ;④同一胎次东方田鼠之间基因共享度大约在 72 %～ 96%之间。结论 RAPD分析能在一定程度上反映出东方田鼠种的特性以及亚种的特异性 。

Objective\ To research genetic type of hybrid field voles from different regions.Methods\ We used 4 10bp random primers to amplify DNA of hybrid field voles from Ningxia and Dongtinghu in China and analyzed the homology of parents and their offsprings.Results\ There were several fragments of genomic DNA shared by all field voles but specific fragments were seen in the parental generation of the two different regions. 4 primers could be used as characteristic RAPD markers to distinguish the subspecies.Parental RAPD markers were found in the offsprings.The homology of field voles of same litter were among 70%-96%.Conclusion\ RAPD could be used to distinguish the species or subspecies of field voles.\;