新生小鼠急性脑缺氧缺血动物模型的建立

The Replication of Acute Hypoxic Ischemic Brain Damaged Animal Model in Baby Mice

目的 建立急性脑缺氧缺血的动物模型。方法 采用足月妊娠小鼠 (查到阴道栓后 19.5d) ,用剖宫手术方法 ,暴露子宫 ,用止血钳阻断双侧子宫动脉血管 ,致使缺氧缺血后脑部发生病理改变。结果 随着阻断子宫血管时间的延长 ,胎鼠的死亡率增高 ,两者具有直线正相关关系 (P <0 .0 5 )。实验组胎鼠体重 (出生至离乳 )增长缓慢 ,胎鼠爬、翻身及对应激刺激等的运动发育明显迟缓 ,脑部的病理改变明显 ,与人的急性脑缺氧缺血的临床表现 ,脑部病理变化及其后遗症是相似的。结论 采用小鼠做急性脑缺氧缺血的动物模型 ,脑缺氧缺血效果明显 ,本模型的建立 ,可用于人的急性脑缺氧缺血的病理学。

Objective To replicate the acute hypoxic ischemic brain damaged animal model.Methods The uterine arteries of full\|term pregnant mice were ligated to interrupt the blood supply by cesarean section.The baby mice with asphyxia were observed for their physical and behavior development and their pathological changes in their brains.Results With prolonging the duration of interrupting the blood supply,the morbidity in fetal mice increased.The relationship between those was positive linear correlation ( P <0.05).The development of body weight and behavior in baby mice was reatrded.The pathologic changes in the brains were apparent,which were similar to the neonatal.Conclusion The animal model can be used for the study of the neonatal hypoxic ischemic brain damage.\;