紫癜性肾炎患儿外周血单个核细胞白细胞介素-13的变化

Changes of interleukin-13 levels in peripheral blood mononuclear cells from children with Henoch-Schonlein purpura nephritis

目的 探讨白细胞介素１３（ＩＬ－ １３） 在紫癜性肾炎（ＨＳＰＮ） 的变化。方法 应用逆转录－ 多聚酶链反应（ＲＴ－ＰＣＲ） 及双抗体夹心ＥＬＩＳＡ 法，分别检测了３０ 例健康儿童和２０ 例ＨＳＰＮ 患儿活动期和恢复期外周血单个核细胞（ＰＢＭＣ） 在植物血凝素（ＰＨＡ） 刺激下ＩＬ－ １３ ｍＲＮＡ和蛋白水平的变化。结果 ①ＨＳＰＮ 活动期ＰＢＭＣ中ＩＬ－１３ ｍＲＮＡ 和蛋白水平均显著高于正常对照组（０ ．４８ ±０ ．１１ ｖｓ０．３６±０．１６ 和４７ ．８９ ±１１ ．６７ ｐｇ／ｍｌｖｓ３５．２２±４．４２ ｐｇ／ｍｌ， Ｐ均＜ ０．０１） ，恢复期与正常对照组相比无显著差异（０．３８±０．１２ ｖｓ０．３６±０ ．１６ 和３５ ．９４ ±５．６０ ｐｇ／ｍｌｖｓ ３５ ．２２±４ ．４２ ｐｇ／ｍｌ，Ｐ均＞ ０．０５）；②临床上表现为蛋白尿型、肾病综合征型及急性肾炎综合征型者，活动期ＰＢＭＣ中ＩＬ－ １３ ｍＲＮＡ和蛋白水平均显著高于正常对照组，而表现为单纯血尿型者与正常对照组相比无显著差异。结论 ＨＳＰＮ 患儿活动期ＩＬ－ １３ 表达异常，且与临床类型有一定的相关关系。

Objective To investigate the changes of interleukin-13(IL-13) in Henoch-Schonlein purpura nephritis(HSPN). Methods The production of IL-13 protein was determined by ELISA and the expression of IL-13 mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR) in phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMC) in 30 healthy children and 20 children with HSPN during their acute phase and remission stage, respectively. Results The levels of IL-13 mRNA and protein were significantly increased in the patients in their acute phase compared with the controls (0.48±0.11 vs 0.36±0.16 and 47.89±11.67 pg/ml vs 35.22±4.42 pg/ml, P<0.01 for both), while in the remission stage the amount of IL-13 decreased to normal levels (0.38±0.12 vs 0.36±0.16 and 35.94±5.60 pg/ml vs 35.22±4.42 pg/ml, P>0.05 for both). The production of IL-13 mRNA and protein in the acute phase of the HSPN patients clinically with proteinuria, nephrotic syndrome or acute glomerulonephritis syndrome was significantly higher than that in the controls. There was no significant difference between the HSPN patients in the acute phase clinically with only isolated hematuria and the controls. Conclusions The expression of IL-13 is abnormal and correlates with the clinical types in children with HSPN.